Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-4
... target sequence and other closely related sequences . Single oligonucleotides of defined sequence are used : • To screen cDNA or genomic DNA libraries , or subclones derived from them , for additional clones containing segments of DNA ...
... target sequence and other closely related sequences . Single oligonucleotides of defined sequence are used : • To screen cDNA or genomic DNA libraries , or subclones derived from them , for additional clones containing segments of DNA ...
Page 11-55
... target . The optimal temperature for hybridization is then determined on the basis of this value . The procedure requires a cloned target sequence that is complementary ( perfectly or imperfectly , depending on the experiment ) to the ...
... target . The optimal temperature for hybridization is then determined on the basis of this value . The procedure requires a cloned target sequence that is complementary ( perfectly or imperfectly , depending on the experiment ) to the ...
Page 14-30
... target sequence from the yield of amplified product in a standard polymerase chain reaction . Recently , however , G. Gilliland , S. Perrin , and H.F. Bunn ( pers . comm . ) have devised a method to measure the overall efficiency of ...
... target sequence from the yield of amplified product in a standard polymerase chain reaction . Recently , however , G. Gilliland , S. Perrin , and H.F. Bunn ( pers . comm . ) have devised a method to measure the overall efficiency of ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml