Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-29
... teriophage . Insertion of foreign DNA sequences into the polycloning site of these vectors can be scored by the appearance of white colonies on plates containing the chromogenic substrate 5 - bromo - 4 - chloro - 3 - indolyl - B - D ...
... teriophage . Insertion of foreign DNA sequences into the polycloning site of these vectors can be scored by the appearance of white colonies on plates containing the chromogenic substrate 5 - bromo - 4 - chloro - 3 - indolyl - B - D ...
Page 10-68
... teriophage DNA - dependent RNA poly- merases . In The enzymes , 3rd edition ( ed . P.D. Boyer ) , vol . 15 , p . 87. Academ- ic Press , New York . Davanloo , P. , A.H. Rosenberg , J.J. Dunn , and F.W. Studier . 1984. Cloning and ...
... teriophage DNA - dependent RNA poly- merases . In The enzymes , 3rd edition ( ed . P.D. Boyer ) , vol . 15 , p . 87. Academ- ic Press , New York . Davanloo , P. , A.H. Rosenberg , J.J. Dunn , and F.W. Studier . 1984. Cloning and ...
Page 13-42
... teriophage M13 or phagemid DNA . This can be done by analyzing an aliquot of each preparation of DNA by electrophoresis through a 1 % agarose gel , using as marker a standard preparation of bacteriophage M13 DNA . Al- though single ...
... teriophage M13 or phagemid DNA . This can be done by analyzing an aliquot of each preparation of DNA by electrophoresis through a 1 % agarose gel , using as marker a standard preparation of bacteriophage M13 DNA . Al- though single ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml