Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-23
... termini with their 3'5 ' exonucleolytic activities and fill in recessed 3 ' - hydroxyl termini with their polymerizing activities . The combina- tion of these activities therefore generates blunt - ended cDNA molecules , which are then ...
... termini with their 3'5 ' exonucleolytic activities and fill in recessed 3 ' - hydroxyl termini with their polymerizing activities . The combina- tion of these activities therefore generates blunt - ended cDNA molecules , which are then ...
Page 10-51
... Termini of DNA The termini of DNA molecules can be radiolabeled enzymatically to yield molecules that may be used in nuclease - S1 mapping of RNA , as radiolabeled primers in primer - extension reactions , and as markers of defined size ...
... Termini of DNA The termini of DNA molecules can be radiolabeled enzymatically to yield molecules that may be used in nuclease - S1 mapping of RNA , as radiolabeled primers in primer - extension reactions , and as markers of defined size ...
Page 13-78
... termini of the DNA . Asymmetric Labeling of Target DNA Double - stranded DNA can be labeled in the following ways : 32 • At its 5 ' termini by bacteriophage T4 polynucleotide kinase and [ y - 32P ] ATP ( see Chapter 11 , pages 11.31 ...
... termini of the DNA . Asymmetric Labeling of Target DNA Double - stranded DNA can be labeled in the following ways : 32 • At its 5 ' termini by bacteriophage T4 polynucleotide kinase and [ y - 32P ] ATP ( see Chapter 11 , pages 11.31 ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml