Proteome Characterization and ProteomicsTimothy D. Veenstra, Richard D. Smith The content of this volume is designed to reach a wide audience, including those involved with relevant technologies such as electrophoresis and mass spectrometry, to those interested in how proteomics can benefit research. A wide range of techniques are discussed, each specifically designed to address different needs in proteomic analysis. The concluding chapter discusses the important issue related to handling large amounts of data accumulated in proteomic studies.
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Results 1-5 of 53
Page vii
... MS-MS Fragmentation of Modified Peptides. . . . . . . . . . . . . . . 196 III. SALSA: A Pattern Recognition ... ESI-MS in Structural and Functional Proteomics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223 III ...
... MS-MS Fragmentation of Modified Peptides. . . . . . . . . . . . . . . 196 III. SALSA: A Pattern Recognition ... ESI-MS in Structural and Functional Proteomics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223 III ...
Page 28
... MS with separation methods such as liquid chromatography for the analysis of biomolecules was achievable with ionization methods such as FAB and especially thermospray, an early cousin of ESI [17]. However, these MS applications were by ...
... MS with separation methods such as liquid chromatography for the analysis of biomolecules was achievable with ionization methods such as FAB and especially thermospray, an early cousin of ESI [17]. However, these MS applications were by ...
Page 30
... ESI MS-1 MS-2 Collision cell Detector 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 m/z 2442.0590 706.3577 569.3118 967.4693 1655.7728 853.4463 1153.5274 275.1135 438.2177 2182.8898 1873.7757 ...
... ESI MS-1 MS-2 Collision cell Detector 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 m/z 2442.0590 706.3577 569.3118 967.4693 1655.7728 853.4463 1153.5274 275.1135 438.2177 2182.8898 1873.7757 ...
Page 31
... ESI-MS and the ease with which online chromatographic and electrophoretic separation methodologies can be interfaced for protein analysis was obvious [21,22]. ESI mass spectra for 66-kDa bovine serum albumin and its 133-kDa dimer form ...
... ESI-MS and the ease with which online chromatographic and electrophoretic separation methodologies can be interfaced for protein analysis was obvious [21,22]. ESI mass spectra for 66-kDa bovine serum albumin and its 133-kDa dimer form ...
Page 32
... (ESI) of molecules for mass spectral characterization. The sample solution is passed through a stainless steel or ... MS analyzers. For example, a singly protonated 30,000-Da protein would yield an m/z of 30,001, making it within the ...
... (ESI) of molecules for mass spectral characterization. The sample solution is passed through a stainless steel or ... MS analyzers. For example, a singly protonated 30,000-Da protein would yield an m/z of 30,001, making it within the ...
Contents
1 | |
25 | |
57 | |
85 | |
Current Strategies for Quantitative Proteomics | 133 |
Proteome Analysis of Posttranslational Modifications | 161 |
Mapping Protein Modifications with Liquid ChromatographyMass Spectrometry and the SALSA Algorithm | 195 |
Emerging Role of Mass Spectrometry in Structural and Functional Proteomics | 217 |
Application of Separation Technologies to Proteomics Research | 249 |
Proteomics of Membrane Proteins | 271 |
Proteomics in Drug Discovery | 309 |
Maximizing the Amount of Protein Samples for Structure Determination | 343 |
Proteomics and Bioinformatics | 353 |
AUTHOR INDEX | 371 |
SUBJECT INDEX | 403 |
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Proteome Characterization and Proteomics Timothy D. Veenstra,Richard D. Smith No preview available - 2003 |
Common terms and phrases
2D-PAGE abundance acid activity addition adducts affinity allows amino AMTs Anal analysis analyzed Anderson application approach Biochem Biol biological capillary cell changes characterization charged Chem chromatography combination compared complex containing corresponding database demonstrated described detected determined developed digestion disease drug effective Electrophoresis elution ESI-MS et al example expression extracted fractions fragmentation FTICR function gene genome glycosylation human identified increase indicated interactions ionization isolated isotopic labeling limited loss mapping Mass Spectrom mass spectrometry measurements membrane proteins methods mixture modifications molecular MS-MS MS/MS observed obtained organism pairs peptides phosphopeptides phosphorylation possible potential predicted present protein protein expression proteome proteome analysis quantitative range relative require residues SALSA sample selected sensitivity separation sequence shown signaling single specific spectra spectrum staining strategy structural studies tags tandem techniques trap tryptic two-dimensional
Popular passages
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Page 338 - Hunt, DF, Henderson, RA, Shabanowitz, J., Sakaguchi, K., Michel, H., Sevilir, N., Cox, AL, Appella, E., and Engelhard, VH Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry.
Page 191 - Brown, PO and Botstein, D. (1999) Exploring the new world of the genome with DNA microarrays.
Page 130 - Gygi, SP, Rist, B., Gerber, SA, Turecek, F., Gelb, MH, and Aebersold, R. (1999). Quantitative analysis of complex protein mixtures using isotope-coded affinity tags.
Page 335 - The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis — preliminary findings. Electrophoresis, 20, 689-700 (2000).
Page 127 - Pacific Northwest Laboratory is operated by Battelle Memorial Institute for the US Department of Energy under Contract DE-AC06-76-RLO 1830.
Page 128 - Wilm, M., Shevchenko, A., Houthaeve, T., Breit, S., Schweigerer, L., Fotsis, T., and Mann, M. (1996). Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry.
Page 336 - An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.