Proteome Characterization and ProteomicsTimothy D. Veenstra, Richard D. Smith The content of this volume is designed to reach a wide audience, including those involved with relevant technologies such as electrophoresis and mass spectrometry, to those interested in how proteomics can benefit research. A wide range of techniques are discussed, each specifically designed to address different needs in proteomic analysis. The concluding chapter discusses the important issue related to handling large amounts of data accumulated in proteomic studies.
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From inside the book
Results 1-5 of 83
Page vii
... Mass Spectral Identification of Phosphopeptides . . . . . . . . . . . 175 IV. Glycosylation ... Spectrometry and the SALSA Algorithm Daniel C. Liebler, BeauT. Hansen, Juliet A. Jones, Hamid Badghisi, and Daniel E. Mason I. Introduction ...
... Mass Spectral Identification of Phosphopeptides . . . . . . . . . . . 175 IV. Glycosylation ... Spectrometry and the SALSA Algorithm Daniel C. Liebler, BeauT. Hansen, Juliet A. Jones, Hamid Badghisi, and Daniel E. Mason I. Introduction ...
Page 2
... Mass spectrometry Protein arrays/chips Fig. 1. Information obtained from genomic and proteomic analysis. Advances in ... spectrometry (Link et al., 1999; Washburn et al., 2001), and single-dimension ultrahigh-resolution capillary liquid ...
... Mass spectrometry Protein arrays/chips Fig. 1. Information obtained from genomic and proteomic analysis. Advances in ... spectrometry (Link et al., 1999; Washburn et al., 2001), and single-dimension ultrahigh-resolution capillary liquid ...
Page 9
... mass spectrometry detects only the most abundant proteins (Gygi et al., 2000), indicating that the 2D-PAGE approach ... spectral analysis. The first strategy advocates the use of multidimensional separations so that the number of ...
... mass spectrometry detects only the most abundant proteins (Gygi et al., 2000), indicating that the 2D-PAGE approach ... spectral analysis. The first strategy advocates the use of multidimensional separations so that the number of ...
Page 10
... mass spectrometry (Link et al., 1999; Washburn et al., 2001). Another novel method currently being evaluated ... spectrometer (Jensen et al., 1999). A key aspect of using FTICR in proteomics is its ability to routinely obtain very high ...
... mass spectrometry (Link et al., 1999; Washburn et al., 2001). Another novel method currently being evaluated ... spectrometer (Jensen et al., 1999). A key aspect of using FTICR in proteomics is its ability to routinely obtain very high ...
Page 18
... mass spectrometry to identify binding proteins or proteins associated with a particular protein complex. A wide variety of affinity tags are being adapted to protein arrays, enabling the implementation of high-throughput screens to ...
... mass spectrometry to identify binding proteins or proteins associated with a particular protein complex. A wide variety of affinity tags are being adapted to protein arrays, enabling the implementation of high-throughput screens to ...
Contents
1 | |
25 | |
57 | |
85 | |
Current Strategies for Quantitative Proteomics | 133 |
Proteome Analysis of Posttranslational Modifications | 161 |
Mapping Protein Modifications with Liquid ChromatographyMass Spectrometry and the SALSA Algorithm | 195 |
Emerging Role of Mass Spectrometry in Structural and Functional Proteomics | 217 |
Application of Separation Technologies to Proteomics Research | 249 |
Proteomics of Membrane Proteins | 271 |
Proteomics in Drug Discovery | 309 |
Maximizing the Amount of Protein Samples for Structure Determination | 343 |
Proteomics and Bioinformatics | 353 |
AUTHOR INDEX | 371 |
SUBJECT INDEX | 403 |
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Proteome Characterization and Proteomics Timothy D. Veenstra,Richard D. Smith No preview available - 2003 |
Common terms and phrases
2D-PAGE abundance acid activity addition adducts affinity allows amino AMTs Anal analysis analyzed Anderson application approach Biochem Biol biological capillary cell changes characterization charged Chem chromatography combination compared complex containing corresponding database demonstrated described detected determined developed digestion disease drug effective Electrophoresis elution ESI-MS et al example expression extracted fractions fragmentation FTICR function gene genome glycosylation human identified increase indicated interactions ionization isolated isotopic labeling limited loss mapping Mass Spectrom mass spectrometry measurements membrane proteins methods mixture modifications molecular MS-MS MS/MS observed obtained organism pairs peptides phosphopeptides phosphorylation possible potential predicted present protein protein expression proteome proteome analysis quantitative range relative require residues SALSA sample selected sensitivity separation sequence shown signaling single specific spectra spectrum staining strategy structural studies tags tandem techniques trap tryptic two-dimensional
Popular passages
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Page 338 - Hunt, DF, Henderson, RA, Shabanowitz, J., Sakaguchi, K., Michel, H., Sevilir, N., Cox, AL, Appella, E., and Engelhard, VH Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry.
Page 191 - Brown, PO and Botstein, D. (1999) Exploring the new world of the genome with DNA microarrays.
Page 130 - Gygi, SP, Rist, B., Gerber, SA, Turecek, F., Gelb, MH, and Aebersold, R. (1999). Quantitative analysis of complex protein mixtures using isotope-coded affinity tags.
Page 335 - The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis — preliminary findings. Electrophoresis, 20, 689-700 (2000).
Page 127 - Pacific Northwest Laboratory is operated by Battelle Memorial Institute for the US Department of Energy under Contract DE-AC06-76-RLO 1830.
Page 128 - Wilm, M., Shevchenko, A., Houthaeve, T., Breit, S., Schweigerer, L., Fotsis, T., and Mann, M. (1996). Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry.
Page 336 - An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.