Proteome Characterization and ProteomicsTimothy D. Veenstra, Richard D. Smith The content of this volume is designed to reach a wide audience, including those involved with relevant technologies such as electrophoresis and mass spectrometry, to those interested in how proteomics can benefit research. A wide range of techniques are discussed, each specifically designed to address different needs in proteomic analysis. The concluding chapter discusses the important issue related to handling large amounts of data accumulated in proteomic studies.
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... point in time. Whereas twodimensional (sodium dodecyl sulfate and isoelectric focusing) polyacrylamide gel electrophoresis has been the mainstay of proteomics analysis, the field is now embracing new techniques such as multidimensional ...
... point in time. Whereas twodimensional (sodium dodecyl sulfate and isoelectric focusing) polyacrylamide gel electrophoresis has been the mainstay of proteomics analysis, the field is now embracing new techniques such as multidimensional ...
Page 26
... isoelectric point provided by 2D-PAGE, is often sufficient to obtain confident protein identification [3,4]. MS-based methods have become the primary technology used to identify proteins. The sensitivity of MS instrumentation allows for ...
... isoelectric point provided by 2D-PAGE, is often sufficient to obtain confident protein identification [3,4]. MS-based methods have become the primary technology used to identify proteins. The sensitivity of MS instrumentation allows for ...
Page 43
... isoelectric point and in the second dimension on the basis of their molecular weight. After staining, typically with ... isoelectric points, and membrane-associated proteins [59]. To circumvent these problems many groups have coupled ...
... isoelectric point and in the second dimension on the basis of their molecular weight. After staining, typically with ... isoelectric points, and membrane-associated proteins [59]. To circumvent these problems many groups have coupled ...
Page 44
... isoelectric focusing (CIEF) has been coupled directly on-line with ESI-MS to characterize simple [63] and complex ... point the most successful has been the MudPit system developed by J. Yates [72, 73]. In the initial demonstration of ...
... isoelectric focusing (CIEF) has been coupled directly on-line with ESI-MS to characterize simple [63] and complex ... point the most successful has been the MudPit system developed by J. Yates [72, 73]. In the initial demonstration of ...
Page 134
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Contents
1 | |
25 | |
57 | |
85 | |
Current Strategies for Quantitative Proteomics | 133 |
Proteome Analysis of Posttranslational Modifications | 161 |
Mapping Protein Modifications with Liquid ChromatographyMass Spectrometry and the SALSA Algorithm | 195 |
Emerging Role of Mass Spectrometry in Structural and Functional Proteomics | 217 |
Application of Separation Technologies to Proteomics Research | 249 |
Proteomics of Membrane Proteins | 271 |
Proteomics in Drug Discovery | 309 |
Maximizing the Amount of Protein Samples for Structure Determination | 343 |
Proteomics and Bioinformatics | 353 |
AUTHOR INDEX | 371 |
SUBJECT INDEX | 403 |
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Proteome Characterization and Proteomics Timothy D. Veenstra,Richard D. Smith No preview available - 2003 |
Common terms and phrases
2D-PAGE abundance acid activity addition adducts affinity allows amino AMTs Anal analysis analyzed Anderson application approach Biochem Biol biological capillary cell changes characterization charged Chem chromatography combination compared complex containing corresponding database demonstrated described detected determined developed digestion disease drug effective Electrophoresis elution ESI-MS et al example expression extracted fractions fragmentation FTICR function gene genome glycosylation human identified increase indicated interactions ionization isolated isotopic labeling limited loss mapping Mass Spectrom mass spectrometry measurements membrane proteins methods mixture modifications molecular MS-MS MS/MS observed obtained organism pairs peptides phosphopeptides phosphorylation possible potential predicted present protein protein expression proteome proteome analysis quantitative range relative require residues SALSA sample selected sensitivity separation sequence shown signaling single specific spectra spectrum staining strategy structural studies tags tandem techniques trap tryptic two-dimensional
Popular passages
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Page 338 - Hunt, DF, Henderson, RA, Shabanowitz, J., Sakaguchi, K., Michel, H., Sevilir, N., Cox, AL, Appella, E., and Engelhard, VH Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry.
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Page 130 - Gygi, SP, Rist, B., Gerber, SA, Turecek, F., Gelb, MH, and Aebersold, R. (1999). Quantitative analysis of complex protein mixtures using isotope-coded affinity tags.
Page 335 - The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis — preliminary findings. Electrophoresis, 20, 689-700 (2000).
Page 127 - Pacific Northwest Laboratory is operated by Battelle Memorial Institute for the US Department of Energy under Contract DE-AC06-76-RLO 1830.
Page 128 - Wilm, M., Shevchenko, A., Houthaeve, T., Breit, S., Schweigerer, L., Fotsis, T., and Mann, M. (1996). Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry.
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