Proteome Characterization and ProteomicsTimothy D. Veenstra, Richard D. Smith The content of this volume is designed to reach a wide audience, including those involved with relevant technologies such as electrophoresis and mass spectrometry, to those interested in how proteomics can benefit research. A wide range of techniques are discussed, each specifically designed to address different needs in proteomic analysis. The concluding chapter discusses the important issue related to handling large amounts of data accumulated in proteomic studies.
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Page viii
... , or Capillary Electrophoresis? . . . . . . . . . . . . 252 V. Multidimensional Separations of Proteins . . . . . . . . . . . . . . . . . 256 VI. On-Column HPLC and CE Protein Concentration . . . . . . . . . . 263 VII. Detection ...
... , or Capillary Electrophoresis? . . . . . . . . . . . . 252 V. Multidimensional Separations of Proteins . . . . . . . . . . . . . . . . . 256 VI. On-Column HPLC and CE Protein Concentration . . . . . . . . . . 263 VII. Detection ...
Page 2
... multidimensional capillary liquid chromatography coupled with tandem mass spectrometry (Link et al., 1999; Washburn et al., 2001), and single-dimension ultrahigh-resolution capillary liquid chromatography in combination with FTICR mass ...
... multidimensional capillary liquid chromatography coupled with tandem mass spectrometry (Link et al., 1999; Washburn et al., 2001), and single-dimension ultrahigh-resolution capillary liquid chromatography in combination with FTICR mass ...
Page 9
... multidimensional separations so that the number of peptides analyzed by the mass spectrometer at any given time is minimized. The fewer the number of peptides within any given spectrum, the greater the increase in overall dynamic range ...
... multidimensional separations so that the number of peptides analyzed by the mass spectrometer at any given time is minimized. The fewer the number of peptides within any given spectrum, the greater the increase in overall dynamic range ...
Page 10
... multidimensional capillary liquid chromatography (LC) coupled with tandem mass spectrometry (Link et al., 1999; Washburn et al., 2001). Another novel method currently being evaluated involves the analysis of global proteolytic digests ...
... multidimensional capillary liquid chromatography (LC) coupled with tandem mass spectrometry (Link et al., 1999; Washburn et al., 2001). Another novel method currently being evaluated involves the analysis of global proteolytic digests ...
Page 23
... multidimensional protein identification technology. Nat. Biotechnol. 19, 242–247. Weekes, J., Wheeler, C. H., Yan, J. X., Weil, J., Eschenhagen, T., Scholtysik, G., and Dunn, M. J. (1999). Bovine dilated cardiomyopathy: Proteomic ...
... multidimensional protein identification technology. Nat. Biotechnol. 19, 242–247. Weekes, J., Wheeler, C. H., Yan, J. X., Weil, J., Eschenhagen, T., Scholtysik, G., and Dunn, M. J. (1999). Bovine dilated cardiomyopathy: Proteomic ...
Contents
1 | |
25 | |
57 | |
85 | |
Current Strategies for Quantitative Proteomics | 133 |
Proteome Analysis of Posttranslational Modifications | 161 |
Mapping Protein Modifications with Liquid ChromatographyMass Spectrometry and the SALSA Algorithm | 195 |
Emerging Role of Mass Spectrometry in Structural and Functional Proteomics | 217 |
Application of Separation Technologies to Proteomics Research | 249 |
Proteomics of Membrane Proteins | 271 |
Proteomics in Drug Discovery | 309 |
Maximizing the Amount of Protein Samples for Structure Determination | 343 |
Proteomics and Bioinformatics | 353 |
AUTHOR INDEX | 371 |
SUBJECT INDEX | 403 |
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Proteome Characterization and Proteomics Timothy D. Veenstra,Richard D. Smith No preview available - 2003 |
Common terms and phrases
2D-PAGE abundance acid activity addition adducts affinity allows amino AMTs Anal analysis analyzed Anderson application approach Biochem Biol biological capillary cell changes characterization charged Chem chromatography combination compared complex containing corresponding database demonstrated described detected determined developed digestion disease drug effective Electrophoresis elution ESI-MS et al example expression extracted fractions fragmentation FTICR function gene genome glycosylation human identified increase indicated interactions ionization isolated isotopic labeling limited loss mapping Mass Spectrom mass spectrometry measurements membrane proteins methods mixture modifications molecular MS-MS MS/MS observed obtained organism pairs peptides phosphopeptides phosphorylation possible potential predicted present protein protein expression proteome proteome analysis quantitative range relative require residues SALSA sample selected sensitivity separation sequence shown signaling single specific spectra spectrum staining strategy structural studies tags tandem techniques trap tryptic two-dimensional
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