Proteome Characterization and ProteomicsTimothy D. Veenstra, Richard D. Smith The content of this volume is designed to reach a wide audience, including those involved with relevant technologies such as electrophoresis and mass spectrometry, to those interested in how proteomics can benefit research. A wide range of techniques are discussed, each specifically designed to address different needs in proteomic analysis. The concluding chapter discusses the important issue related to handling large amounts of data accumulated in proteomic studies.
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Timothy D. Veenstra, Richard D. Smith. This book is printed on acid-free paper ... on the condition, however, that the copier pay the stated per copy fee ... line via the Elsevier Science homepage (http://elsevier.com), by selecting ...
Timothy D. Veenstra, Richard D. Smith. This book is printed on acid-free paper ... on the condition, however, that the copier pay the stated per copy fee ... line via the Elsevier Science homepage (http://elsevier.com), by selecting ...
Page 10
... online separations. The MMA that can be routinely obtained with FTICR instrumentation is potentially $0.1 ppm (although much more accurate measurements have been demonstrated for specialized applications), which is much higher than the ...
... online separations. The MMA that can be routinely obtained with FTICR instrumentation is potentially $0.1 ppm (although much more accurate measurements have been demonstrated for specialized applications), which is much higher than the ...
Page 13
... online with MS. Two experimental MS strategies (MS and tandem MS) are used to identify and quantify the phosphorylation state of the phosphopeptides. In the MS mode, the masses of the intact peptide pairs are measured and the relative ...
... online with MS. Two experimental MS strategies (MS and tandem MS) are used to identify and quantify the phosphorylation state of the phosphopeptides. In the MS mode, the masses of the intact peptide pairs are measured and the relative ...
Page 31
... online chromatographic and electrophoretic separation methodologies can be interfaced for protein analysis was obvious ... on exiting the needle. Analyte ions, as well as ions originating from other solutes, are contained within these ...
... online chromatographic and electrophoretic separation methodologies can be interfaced for protein analysis was obvious ... on exiting the needle. Analyte ions, as well as ions originating from other solutes, are contained within these ...
Page 42
... on opposite sides of the trap. Energy can also be applied to dissociate the ... on opposite sides of the trap. The energy applied can also be used to ... line separations such as reversed-phase LC [57] or capillary electrophoresis ...
... on opposite sides of the trap. Energy can also be applied to dissociate the ... on opposite sides of the trap. The energy applied can also be used to ... line separations such as reversed-phase LC [57] or capillary electrophoresis ...
Contents
1 | |
25 | |
57 | |
85 | |
Current Strategies for Quantitative Proteomics | 133 |
Proteome Analysis of Posttranslational Modifications | 161 |
Mapping Protein Modifications with Liquid ChromatographyMass Spectrometry and the SALSA Algorithm | 195 |
Emerging Role of Mass Spectrometry in Structural and Functional Proteomics | 217 |
Application of Separation Technologies to Proteomics Research | 249 |
Proteomics of Membrane Proteins | 271 |
Proteomics in Drug Discovery | 309 |
Maximizing the Amount of Protein Samples for Structure Determination | 343 |
Proteomics and Bioinformatics | 353 |
AUTHOR INDEX | 371 |
SUBJECT INDEX | 403 |
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Proteome Characterization and Proteomics Timothy D. Veenstra,Richard D. Smith No preview available - 2003 |
Common terms and phrases
2D-PAGE abundance acid activity addition adducts affinity allows amino AMTs Anal analysis analyzed Anderson application approach Biochem Biol biological capillary cell changes characterization charged Chem chromatography combination compared complex containing corresponding database demonstrated described detected determined developed digestion disease drug effective Electrophoresis elution ESI-MS et al example expression extracted fractions fragmentation FTICR function gene genome glycosylation human identified increase indicated interactions ionization isolated isotopic labeling limited loss mapping Mass Spectrom mass spectrometry measurements membrane proteins methods mixture modifications molecular MS-MS MS/MS observed obtained organism pairs peptides phosphopeptides phosphorylation possible potential predicted present protein protein expression proteome proteome analysis quantitative range relative require residues SALSA sample selected sensitivity separation sequence shown signaling single specific spectra spectrum staining strategy structural studies tags tandem techniques trap tryptic two-dimensional
Popular passages
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Page 338 - Hunt, DF, Henderson, RA, Shabanowitz, J., Sakaguchi, K., Michel, H., Sevilir, N., Cox, AL, Appella, E., and Engelhard, VH Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry.
Page 191 - Brown, PO and Botstein, D. (1999) Exploring the new world of the genome with DNA microarrays.
Page 130 - Gygi, SP, Rist, B., Gerber, SA, Turecek, F., Gelb, MH, and Aebersold, R. (1999). Quantitative analysis of complex protein mixtures using isotope-coded affinity tags.
Page 335 - The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis — preliminary findings. Electrophoresis, 20, 689-700 (2000).
Page 127 - Pacific Northwest Laboratory is operated by Battelle Memorial Institute for the US Department of Energy under Contract DE-AC06-76-RLO 1830.
Page 128 - Wilm, M., Shevchenko, A., Houthaeve, T., Breit, S., Schweigerer, L., Fotsis, T., and Mann, M. (1996). Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry.
Page 336 - An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.