Molecular cloning: a laboratory manual. Vol. 1, Volume 3

Acad activity aliquots antibody Appendix appropriate assay B-galactosidase bacterial bacteriophage bacteriophage T7 baculovirus bait binding Biochem Biol buffer cDNA cell lines cell lysate centrifugation Chapter chloramphenicol cleavage cloning colonies concentration containing culture detection Dilute dishes dithiothreitol DNA polymerase DNase double-stranded EDTA efficiency electrophoresis electroporation encoding enzyme epitope Escherichia coli ethanol eukaryotic extract fluorescent protein foreign protein fragment fusion protein gene expression genomic glycerol green fluorescent protein H₂O hybridization inclusion bodies Incubate induced interactions lacZ luciferase lysis mammalian cells medium membrane method mg/ml minutes molecules mutations Natl nuclear Nucleic Acids pellet peptide plasmid plates probe Proc promoter protease Protocol purification radiolabeled reaction reagents recombinant repressor Resuspend room temperature rotor SDS-polyacrylamide gel selection sequence Sorvall ẞ-galactosidase Step sterile stock solutions strain supernatant target gene target protein tein terminus tetracycline tion transcription transfected cells transfection transfer transformation Tris-Cl pH two-hybrid wash yeast µg/ml