Molecular Biology Problem Solver: A Laboratory GuideMost research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:
A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:
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Page 21
... biochemical has been kept at proper storage conditions , it is a good idea to determine the approximate age of the chemical or biochemical . It is risky to use a reagent whose age can't be determined . Manufacturers may or may not have ...
... biochemical has been kept at proper storage conditions , it is a good idea to determine the approximate age of the chemical or biochemical . It is risky to use a reagent whose age can't be determined . Manufacturers may or may not have ...
Page 32
... biochemical reactions (whose products might be used in subsequent biochemical reactions). Complex buffer systems are used in electrophoretic systems to control pH or establish pH gradients. Can You Substitute One Buffer for Another? It ...
... biochemical reactions (whose products might be used in subsequent biochemical reactions). Complex buffer systems are used in electrophoretic systems to control pH or establish pH gradients. Can You Substitute One Buffer for Another? It ...
Page 35
... Biochemical Compatibility Is the buffer applied at an early stage of a research project com- patible with a downstream step? A protein isolated in a buffer containing 10mM Mg2+ appears innocuous, but this cation con- centration could ...
... Biochemical Compatibility Is the buffer applied at an early stage of a research project com- patible with a downstream step? A protein isolated in a buffer containing 10mM Mg2+ appears innocuous, but this cation con- centration could ...
Page 43
... biochemistry department when the first mention of a problem with the house distilled water was a memo that came out from the maintenance department that stated : " We would like to inform you that the repairs have been made to the still ...
... biochemistry department when the first mention of a problem with the house distilled water was a memo that came out from the maintenance department that stated : " We would like to inform you that the repairs have been made to the still ...
Page 110
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Contents
31 | |
Chapter 4 How to Properly Use and Maintain Laboratory Equipment | 49 |
Chapter 5 Working Safely with Biological Samples | 113 |
Chapter 6 Working Safely with Radioactive Materials | 141 |
Chapter 7 DNA Purification | 167 |
Chapter 8 RNA Purification | 197 |
Chapter 9 Restriction Endonucleases | 225 |
Chapter 10 Nucleotides Oligonucleotides and Polynucleotides | 267 |
Chapter 11 PCR | 291 |
Chapter 12 Electrophoresis | 331 |
Chapter 13 Western Blotting | 373 |
Chapter 14 Nucleic Acid Hybridization | 399 |
Chapter 15 E coli Expression Systems | 461 |
Chapter 16 Eukaryotic Expression | 491 |
Index | 543 |
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Common terms and phrases
absorbance Acids Res acrylamide activity agarose Amersham Amersham Pharmacia Biotech amplification antibody applications assay autoclave baculovirus binding Biochem blot calibration cDNA cell lines centrifugation cleavage cloning codon coli concentration containing denaturing detection digestion dilution DNA polymerase efficiency electrode electrophoresis Escherichia coli expression expression vector filling solution filters gene genomic DNA gradient increase incubation infection insect cells labeled liquid lysis manufacturer material measured membrane meter method methylase molecular weight molecules nitrocellulose nonradioactive Nucl nucleic acids nucleotide oligonucleotide optimal pellet Pharmacia pipette plasmid polymer preparation primer probe problem procedure protease protein of interest protocols purification quantitative radioactive reaction reagents recombinant resin restriction endonuclease restriction enzyme rotor salt sample screen SDS-PAGE secondary antibody sensitivity sequence signal specific stability stain standard storage phosphor stored strategy streptavidin suppliers target temperature template tion tissue transfer tube vector volume wash Western blot
Popular passages
Page 325 - Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.
Page 191 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Page 223 - Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
Page 191 - PM, and van der Noordaa, J. (1990) Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28, 495-503.
Page 539 - Berg, P. (1981). Selection for animal cells that express the escherichia coli gene coding for xanthineguanine phosphoribosyltransferase. Proc Natl Acad Sci USA 78, 2072-2076.
Page 191 - Birnboim, HC and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Page 322 - Akane, A., Matsubara, K., Nakamura, H., Takahashi, S., and Kimura, K. (1994) Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification.
Page 323 - Cheng, S., Fockler, C., Barnes, WM, and Higuchi, R (1994) Effective amplification of long targets from cloned inserts and human genomic DNA.