Molecular Biology Problem Solver: A Laboratory GuideMost research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:
A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:
|
From inside the book
Results 1-5 of 44
Page x
... Pharmacia Biotech for their support, good wishes and collective sense of humor. With such a talented group of supporters and contributing authors, you can blame me for any inadequacies you note within these pages. There are probably ...
... Pharmacia Biotech for their support, good wishes and collective sense of humor. With such a talented group of supporters and contributing authors, you can blame me for any inadequacies you note within these pages. There are probably ...
Page xi
... CT, support@packardinstrument.com Franciskovich, Phillip P., Motorola Life Sciences, Tempe AZ, apf008@email.mot.com Gerstein , Alan S. , Amersham Pharmacia Biotech , Piscataway xi MOLECULAR BIOLOGY PROBLEM SOLVER: Contributors.
... CT, support@packardinstrument.com Franciskovich, Phillip P., Motorola Life Sciences, Tempe AZ, apf008@email.mot.com Gerstein , Alan S. , Amersham Pharmacia Biotech , Piscataway xi MOLECULAR BIOLOGY PROBLEM SOLVER: Contributors.
Page xii
... Pharmacia Biotech , Piscataway , NJ , sibylle.herzer@am.apbiotech.com Kennedy , Michele A. , Brinkmann Instruments , Inc. , Westbury , NY , info@brinkmann.com Kirkpatrick , Robert , GlaxoSmithKline , King of Prussia , PA Kracklauer ...
... Pharmacia Biotech , Piscataway , NJ , sibylle.herzer@am.apbiotech.com Kennedy , Michele A. , Brinkmann Instruments , Inc. , Westbury , NY , info@brinkmann.com Kirkpatrick , Robert , GlaxoSmithKline , King of Prussia , PA Kracklauer ...
Page xiii
... .com Volny , William R. J. Jr. , Amersham Pharmacia Biotech , Piscataway , NJ Walsh , Paul R. , New England Biolabs , Beverly , MA Preparing for Success in the Laboratory Phillip P. Franciskovich The Contributors xiii.
... .com Volny , William R. J. Jr. , Amersham Pharmacia Biotech , Piscataway , NJ Walsh , Paul R. , New England Biolabs , Beverly , MA Preparing for Success in the Laboratory Phillip P. Franciskovich The Contributors xiii.
Page xiii
... , Milwaukee,WI, Tom.tyre@piercenet.com Volny,William R.J. Jr.,Amersham Pharmacia Biotech, Piscataway, NJ Walsh, Paul R., New England Biolabs, Beverly, MA Phillip P. Franciskovich The Project . . . . . Contributors xiii.
... , Milwaukee,WI, Tom.tyre@piercenet.com Volny,William R.J. Jr.,Amersham Pharmacia Biotech, Piscataway, NJ Walsh, Paul R., New England Biolabs, Beverly, MA Phillip P. Franciskovich The Project . . . . . Contributors xiii.
Contents
31 | |
Chapter 4 How to Properly Use and Maintain Laboratory Equipment | 49 |
Chapter 5 Working Safely with Biological Samples | 113 |
Chapter 6 Working Safely with Radioactive Materials | 141 |
Chapter 7 DNA Purification | 167 |
Chapter 8 RNA Purification | 197 |
Chapter 9 Restriction Endonucleases | 225 |
Chapter 10 Nucleotides Oligonucleotides and Polynucleotides | 267 |
Chapter 11 PCR | 291 |
Chapter 12 Electrophoresis | 331 |
Chapter 13 Western Blotting | 373 |
Chapter 14 Nucleic Acid Hybridization | 399 |
Chapter 15 E coli Expression Systems | 461 |
Chapter 16 Eukaryotic Expression | 491 |
Index | 543 |
Other editions - View all
Common terms and phrases
absorbance Acids Res acrylamide activity agarose Amersham Amersham Pharmacia Biotech amplification antibody applications assay autoclave baculovirus binding Biochem blot calibration cDNA cell lines centrifugation cleavage cloning codon coli concentration containing denaturing detection digestion dilution DNA polymerase efficiency electrode electrophoresis Escherichia coli expression expression vector filling solution filters gene genomic DNA gradient increase incubation infection insect cells labeled liquid lysis manufacturer material measured membrane meter method methylase molecular weight molecules nitrocellulose nonradioactive Nucl nucleic acids nucleotide oligonucleotide optimal pellet Pharmacia pipette plasmid polymer preparation primer probe problem procedure protease protein of interest protocols purification quantitative radioactive reaction reagents recombinant resin restriction endonuclease restriction enzyme rotor salt sample screen SDS-PAGE secondary antibody sensitivity sequence signal specific stability stain standard storage phosphor stored strategy streptavidin suppliers target temperature template tion tissue transfer tube vector volume wash Western blot
Popular passages
Page 325 - Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.
Page 191 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Page 223 - Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
Page 191 - PM, and van der Noordaa, J. (1990) Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28, 495-503.
Page 539 - Berg, P. (1981). Selection for animal cells that express the escherichia coli gene coding for xanthineguanine phosphoribosyltransferase. Proc Natl Acad Sci USA 78, 2072-2076.
Page 191 - Birnboim, HC and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Page 322 - Akane, A., Matsubara, K., Nakamura, H., Takahashi, S., and Kimura, K. (1994) Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification.
Page 323 - Cheng, S., Fockler, C., Barnes, WM, and Higuchi, R (1994) Effective amplification of long targets from cloned inserts and human genomic DNA.