Molecular Biology Problem Solver: A Laboratory GuideMost research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:
A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:
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Page 34
... bind copper. The presence of significant levels of organic solvents can limit solubility of some inorganic buffers. Potassium phosphate, for example, is more readily soluble in some organic solutions than the corresponding sodium ...
... bind copper. The presence of significant levels of organic solvents can limit solubility of some inorganic buffers. Potassium phosphate, for example, is more readily soluble in some organic solutions than the corresponding sodium ...
Page 35
... binding of amine-type buffers (i.e., Tris) to a silica-based chromatography packing. Biochemical Compatibility Is the buffer applied at an early stage of a research project compatible with a downstream step? A protein isolated in a ...
... binding of amine-type buffers (i.e., Tris) to a silica-based chromatography packing. Biochemical Compatibility Is the buffer applied at an early stage of a research project compatible with a downstream step? A protein isolated in a ...
Page 37
... binds with high affinity to one of the solution components. This is usually not as problematic with polar buffer salts as it can be with cofactors, vitamins, and the like. This effect is very clearly demonstrated when a solution is ...
... binds with high affinity to one of the solution components. This is usually not as problematic with polar buffer salts as it can be with cofactors, vitamins, and the like. This effect is very clearly demonstrated when a solution is ...
Page 109
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Contents
ix | |
xi | |
1 | |
11 | |
31 | |
49 | |
Chapter 5 Working Safely with Biological Samples | 113 |
Chapter 6 Working Safely with Radioactive Materials | 141 |
Chapter 9 Restriction Endonucleases | 225 |
Chapter 10 Nucleotides Oligonucleotides and Polynucleotides | 267 |
Chapter 11 PCR | 291 |
Chapter 12 Electrophoresis | 331 |
Chapter 13 Western Blotting | 373 |
Chapter 14 Nucleic Acid Hybridization | 399 |
Chapter 15 E coli Expression Systems | 461 |
Chapter 16 Eukaryotic Expression | 491 |
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Common terms and phrases
absorbance Acids Res acrylamide activity agarose Amersham Amersham Pharmacia Biotech amplification antibody applications assay autoclave baculovirus binding Biochem blot calibration cDNA cell lines centrifugation cleavage cloning codon coli concentration containing cuvette denaturing detection digestion dilution DNA polymerase efficiency electrode electrophoresis Escherichia coli expression expression vector filling solution filter gene genomic DNA gradient increase incubation infection labeled Laboratory liquid lysis manufacturer material measured membrane meter method methylase molecular weight molecules nitrocellulose nonradioactive nucleic acids nucleotide oligonucleotide optimal pellet Pharmacia pipette plasmid polymer preparation primer probe problem procedure protease protein of interest protocols purification quantitative radioactive reaction reagents recombinant resin restriction endonuclease restriction enzyme rotor salt sample screen SDS-PAGE secondary antibody sensitivity sequence signal specific stability stain standard storage phosphor stored strategy streptavidin suppliers target temperature template tion tissue transfer tube vector volume wash Western blot
Popular passages
Page 324 - Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.
Page 190 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Page 222 - Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
Page 190 - PM, and van der Noordaa, J. (1990) Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28, 495-503.
Page 538 - Berg, P. (1981). Selection for animal cells that express the escherichia coli gene coding for xanthineguanine phosphoribosyltransferase. Proc Natl Acad Sci USA 78, 2072-2076.
Page 190 - Birnboim, HC and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Page 321 - Akane, A., Matsubara, K., Nakamura, H., Takahashi, S., and Kimura, K. (1994) Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification.
Page 322 - Cheng, S., Fockler, C., Barnes, WM, and Higuchi, R (1994) Effective amplification of long targets from cloned inserts and human genomic DNA.