Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 92
... angle meridional streaks or reflections near the center of the diffraction pat- tern at diffraction angles less than about 4 ° . The layer lines on which these streaks occur are close together because of the large repeat distance ...
... angle meridional streaks or reflections near the center of the diffraction pat- tern at diffraction angles less than about 4 ° . The layer lines on which these streaks occur are close together because of the large repeat distance ...
Page 180
... angle B. For a collec- tion of such systems the degree of polarization of fluorescence at 90 ° to the incident beam is determined by the average value of the angle between the direction of the absorption oscillator at the moment of ...
... angle B. For a collec- tion of such systems the degree of polarization of fluorescence at 90 ° to the incident beam is determined by the average value of the angle between the direction of the absorption oscillator at the moment of ...
Page 182
... angle ẞ between the directions of the two oscillators and , according to the theory of Jablonski ( 1935 ) , is given ... angle during the interval between excitation and emission . The average value of this angle will depend upon the ...
... angle ẞ between the directions of the two oscillators and , according to the theory of Jablonski ( 1935 ) , is given ... angle during the interval between excitation and emission . The average value of this angle will depend upon the ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone