Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 83
... atomic scattering factors of the two atoms . Figure 16a shows how the map for the pleated sheet would be modi- fied if potassium atoms were present at the positions marked by crosses in Fig . 15b . The strongest peaks contain the ...
... atomic scattering factors of the two atoms . Figure 16a shows how the map for the pleated sheet would be modi- fied if potassium atoms were present at the positions marked by crosses in Fig . 15b . The strongest peaks contain the ...
Page 85
... atoms and O atoms . ( c ) , Improved map using phases calculated for heavy atoms plus the Ca , C , and O atoms in the approximate posi- tions shown in ( b ) . All atoms are now clearly resolved and further refinement either by Fourier ...
... atoms and O atoms . ( c ) , Improved map using phases calculated for heavy atoms plus the Ca , C , and O atoms in the approximate posi- tions shown in ( b ) . All atoms are now clearly resolved and further refinement either by Fourier ...
Page 87
... atoms to certain reflections will be too small to resolve the ambiguity . Because of experimental errors ... atoms in the unit cell . For a given pair of structure amplitudes FP and FPH for the protein and protein - plus - heavy - atoms ...
... atoms to certain reflections will be too small to resolve the ambiguity . Because of experimental errors ... atoms in the unit cell . For a given pair of structure amplitudes FP and FPH for the protein and protein - plus - heavy - atoms ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone