Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 232
... axial ratio for rigid ellipsoids of revolution . The frictional ratio f / f . obtainable by hydrodynamic methods also increases with molecular asymmetry . There is , however , an important difference between the range of values to be ...
... axial ratio for rigid ellipsoids of revolution . The frictional ratio f / f . obtainable by hydrodynamic methods also increases with molecular asymmetry . There is , however , an important difference between the range of values to be ...
Page 315
... ratio of the molecular axes . Likewise , the rotational diffusion constant along the b axis ( around the a axis ) is ... axial ratio alone . Consequently , if we assume a certain value for the ratio A / A € 2 and for the molecular axes ...
... ratio of the molecular axes . Likewise , the rotational diffusion constant along the b axis ( around the a axis ) is ... axial ratio alone . Consequently , if we assume a certain value for the ratio A / A € 2 and for the molecular axes ...
Page 316
... axial ratio and the dipole angle . The axial ratios of various proteins thus determined are in reasonable agreement with the values obtained using other methods . Oncley's theory is based on the assumption that proteins have a permanent ...
... axial ratio and the dipole angle . The axial ratios of various proteins thus determined are in reasonable agreement with the values obtained using other methods . Oncley's theory is based on the assumption that proteins have a permanent ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone