Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 3
... beam of accelerated electrons . Electrons produced by thermal emission from a tungsten filament are accelerated through voltages of the order of 100 kV , forming the illuminating beam . The equivalent electron wavelength , determined by ...
... beam of accelerated electrons . Electrons produced by thermal emission from a tungsten filament are accelerated through voltages of the order of 100 kV , forming the illuminating beam . The equivalent electron wavelength , determined by ...
Page 62
... beam . Instead its intensity would fluctuate , being large in directions in which the waves scattered by the individual atoms were approximately in step . If we rotated the molecule , the pattern of inten- sity would vary according to ...
... beam . Instead its intensity would fluctuate , being large in directions in which the waves scattered by the individual atoms were approximately in step . If we rotated the molecule , the pattern of inten- sity would vary according to ...
Page 211
... beam of light . c . Two photomultiplier tubes for monitoring the fluorescent and ref- erence beams . d . A compensating device for altering the phase . e . A detector , usually a radio receiver . Since very high frequencies of ...
... beam of light . c . Two photomultiplier tubes for monitoring the fluorescent and ref- erence beams . d . A compensating device for altering the phase . e . A detector , usually a radio receiver . Since very high frequencies of ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone