Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 337
... birefringence . Chaudier ( 1908 ) studied the elec- tric birefringence of finely powdered crystals suspended in organic liquids . Diesselhorst and Freundlich ( 1915 ) found that vanadium pent- oxide sols became birefringent in an ...
... birefringence . Chaudier ( 1908 ) studied the elec- tric birefringence of finely powdered crystals suspended in organic liquids . Diesselhorst and Freundlich ( 1915 ) found that vanadium pent- oxide sols became birefringent in an ...
Page 344
... birefringence is given by Δη = Ano exp ( −60t ) ( 22 ) where An is the birefringence at any time after the commencement of decay and An , is the birefringence at time t = 0 ( Benoit , 1951 ) . The relaxation time 7 , defined as the ...
... birefringence is given by Δη = Ano exp ( −60t ) ( 22 ) where An is the birefringence at any time after the commencement of decay and An , is the birefringence at time t = 0 ( Benoit , 1951 ) . The relaxation time 7 , defined as the ...
Page 358
... birefringent in an electric field . Subsequently , Lauffer ( 1939 ) investigated the electric birefringence in an alternating field of 60 cycles / sec . Since the electric birefringence , as well as the flow birefringence , of dilute ...
... birefringent in an electric field . Subsequently , Lauffer ( 1939 ) investigated the electric birefringence in an alternating field of 60 cycles / sec . Since the electric birefringence , as well as the flow birefringence , of dilute ...
Contents
Electron Microscopy of Globular Proteins | 2 |
The Enhancement of Contrast | 21 |
The Preservation of Specimens | 35 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone