Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 151
... determined mainly from the absorption near 295 mμ , where the ab- sorption of cystine is less , and that of ionized tyrosine more , than at 280 mμ . ) The method of Edelhoch is better suited for the determination of tryptophan M Trp ...
... determined mainly from the absorption near 295 mμ , where the ab- sorption of cystine is less , and that of ionized tyrosine more , than at 280 mμ . ) The method of Edelhoch is better suited for the determination of tryptophan M Trp ...
Page 163
... determined . If the perturbant produces an increase in absorption , the contents of the two compartments of the ... determined by the thickness of a gasket of inert material are also available commercially . The actual path length of ...
... determined . If the perturbant produces an increase in absorption , the contents of the two compartments of the ... determined by the thickness of a gasket of inert material are also available commercially . The actual path length of ...
Page 483
... determined readily from Eq . ( 8 ) , and hence quantitative interpretation is feasible if VA and Vp , the respective monomer and polymer elution volumes , are also known ( Ackers and Thompson , 1965 ) . V is determined from each of a ...
... determined readily from Eq . ( 8 ) , and hence quantitative interpretation is feasible if VA and Vp , the respective monomer and polymer elution volumes , are also known ( Ackers and Thompson , 1965 ) . V is determined from each of a ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone