Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 166
... disulfide bond . Because of repulsion between 3pπ orbitals of the sulfur atoms , the disulfide chromophore has a dihedral angle of 90 ° in the unstrained condition . Since two nonsuper- posable forms can be constructed , and because the ...
... disulfide bond . Because of repulsion between 3pπ orbitals of the sulfur atoms , the disulfide chromophore has a dihedral angle of 90 ° in the unstrained condition . Since two nonsuper- posable forms can be constructed , and because the ...
Page 235
... disulfide bonds , which produced only small , instantaneous effects on viscosity and optical rotation . Only in 6 M guanidine solutions were the polarization values comparable with those reported for homologous polypeptides in their ...
... disulfide bonds , which produced only small , instantaneous effects on viscosity and optical rotation . Only in 6 M guanidine solutions were the polarization values comparable with those reported for homologous polypeptides in their ...
Page 523
... disulfide bonds , see Disulfide bonds of tryptophan , see N - Bromosuccin- imide P Palladium , use of , in shadow casting , 23 Papain , digestion of myosin by , 53 Paper crossing- , electrophoresis , 440-441 electrophoresis on , 422-423 ...
... disulfide bonds , see Disulfide bonds of tryptophan , see N - Bromosuccin- imide P Palladium , use of , in shadow casting , 23 Papain , digestion of myosin by , 53 Paper crossing- , electrophoresis , 440-441 electrophoresis on , 422-423 ...
Contents
Electron Microscopy of Globular Proteins | 2 |
The Enhancement of Contrast | 21 |
The Preservation of Specimens | 35 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone