Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 369
... Electrophoresis 369 371 373 379 A. Principles of Moving - Boundary Electrophoresis B. The Tiselius Electrophoresis Apparatus and Procedures 379 381 C. Analysis and Interpretation of Electrophoretic Patterns D. Electrophoresis as a ...
... Electrophoresis 369 371 373 379 A. Principles of Moving - Boundary Electrophoresis B. The Tiselius Electrophoresis Apparatus and Procedures 379 381 C. Analysis and Interpretation of Electrophoretic Patterns D. Electrophoresis as a ...
Page 379
... Electrophoresis Of the several techniques available for electrophoretic analysis , the moving - boundary method is the only one which permits highly precise physicochemical ... ELECTROPHORESIS 379 Moving-Boundary Electrophoresis.
... Electrophoresis Of the several techniques available for electrophoretic analysis , the moving - boundary method is the only one which permits highly precise physicochemical ... ELECTROPHORESIS 379 Moving-Boundary Electrophoresis.
Page 409
... electrophoresis cell , dialyzed against buffer , and subjected to electrophoresis without restor- ing the protein concentration used in the original separation , the ascend- ing as well as the descending pattern of the fraction might ...
... electrophoresis cell , dialyzed against buffer , and subjected to electrophoresis without restor- ing the protein concentration used in the original separation , the ascend- ing as well as the descending pattern of the fraction might ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone