Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 319
... experimental difficulties , there is con- siderable experimental scatter but this does not obscure the significance of the plot . The disparity between the experimental result and the theoretical prediction by Kirkwood and Shumaker is ...
... experimental difficulties , there is con- siderable experimental scatter but this does not obscure the significance of the plot . The disparity between the experimental result and the theoretical prediction by Kirkwood and Shumaker is ...
Page 358
... Experimental Results The experimental methods outlined above have been applied to bio- logical polymers ( proteins , nucleic acids , nucleoproteins , ribosomes , viruses , etc. ) , synthetic polymers ( polypeptides , polynucleotides ...
... Experimental Results The experimental methods outlined above have been applied to bio- logical polymers ( proteins , nucleic acids , nucleoproteins , ribosomes , viruses , etc. ) , synthetic polymers ( polypeptides , polynucleotides ...
Page 457
... Experimental Aspects The aim of this section is to outline generally the various experimental techniques , and to assess the nature and adequacy of the assumptions inherent in a particular method . Emphasis is placed on the theoretical ...
... Experimental Aspects The aim of this section is to outline generally the various experimental techniques , and to assess the nature and adequacy of the assumptions inherent in a particular method . Emphasis is placed on the theoretical ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone