Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 71
... Factor Reflections from crystals can occur only if all the unit cells scatter in phase and the structure factor for a particular reflection is a complex 8 7 Oxygen 6 5 f 4 3 2 0 I 0.5 Carbon Carbon with temperature factor 1.0 d * ( Å1 ) ...
... Factor Reflections from crystals can occur only if all the unit cells scatter in phase and the structure factor for a particular reflection is a complex 8 7 Oxygen 6 5 f 4 3 2 0 I 0.5 Carbon Carbon with temperature factor 1.0 d * ( Å1 ) ...
Page 78
... factor is introduced . To minimize loss of resolution , the data may be multiplied by a positive exponential and this process is called artificial sharpening ( Dickerson et al . , 1961 ) . It can be applied only to a limited extent ...
... factor is introduced . To minimize loss of resolution , the data may be multiplied by a positive exponential and this process is called artificial sharpening ( Dickerson et al . , 1961 ) . It can be applied only to a limited extent ...
Page 339
... factor , 91-92 , and an orientation factor , , defined in Eq . ( 5 ) . The optical anisotropy factor is the difference between the excess optical polariza- bility per unit volume of the macromolecule along the symmetry axis and that ...
... factor , 91-92 , and an orientation factor , , defined in Eq . ( 5 ) . The optical anisotropy factor is the difference between the excess optical polariza- bility per unit volume of the macromolecule along the symmetry axis and that ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone