Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 451
Sydney J. Leach. 9 Analytical Gel Filtration D. J. WINZOR Glossary of Symbols I. Introduction II . Theory of Gel Filtration A. General Chromatographic Representation . B. Specific Theories of Gel Filtration III . Experimental Aspects A. Gel ...
Sydney J. Leach. 9 Analytical Gel Filtration D. J. WINZOR Glossary of Symbols I. Introduction II . Theory of Gel Filtration A. General Chromatographic Representation . B. Specific Theories of Gel Filtration III . Experimental Aspects A. Gel ...
Page 453
... Gel filtration and ultracentrifugation are both transport procedures in which migration is governed largely by the size of the solute under investigation , and results obtained by the two ... GEL FILTRATION 453 Theory of Gel Filtration.
... Gel filtration and ultracentrifugation are both transport procedures in which migration is governed largely by the size of the solute under investigation , and results obtained by the two ... GEL FILTRATION 453 Theory of Gel Filtration.
Page 457
... gel filtration are readily available from , e.g. , the reviews by Flodin ( 1962 ) and Andrews ( 1966 ) . A. GEL FILTRATION MEDIA The types of gel currently in use with aqueous solvent systems have been tabulated by Andrews ( 1966 ) . Of ...
... gel filtration are readily available from , e.g. , the reviews by Flodin ( 1962 ) and Andrews ( 1966 ) . A. GEL FILTRATION MEDIA The types of gel currently in use with aqueous solvent systems have been tabulated by Andrews ( 1966 ) . Of ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone