Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 127
... group . At 234 mu , Ae for the carboxyl dissociation is negative , and that for the amino group positive , while at 228 mu , both groups produce a positive Ae . No absorption changes at all are observed at 287 mu , an isosbestic point ...
... group . At 234 mu , Ae for the carboxyl dissociation is negative , and that for the amino group positive , while at 228 mu , both groups produce a positive Ae . No absorption changes at all are observed at 287 mu , an isosbestic point ...
Page 135
... groups oxidized . When three indole groups in a - chymotrypsino- gen have been oxidized , the chromophore exposure falls to zero , indicat- ing that only these three indole groups were exposed to solvent . In lysozyme , oxidation of all ...
... groups oxidized . When three indole groups in a - chymotrypsino- gen have been oxidized , the chromophore exposure falls to zero , indicat- ing that only these three indole groups were exposed to solvent . In lysozyme , oxidation of all ...
Page 145
... groups is 286.0 — 0.3 = 285.7 mμ , which is 0.7 mu to the red of the model compound acetyl- tyrosine ethyl ester ... groups are titrated , it is possible that this is an example of a direct charge effect of an imidazole group upon a ...
... groups is 286.0 — 0.3 = 285.7 mμ , which is 0.7 mu to the red of the model compound acetyl- tyrosine ethyl ester ... groups are titrated , it is possible that this is an example of a direct charge effect of an imidazole group upon a ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone