Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 3
... light microscope , in which light rays are replaced by a beam of accelerated electrons . Electrons produced by thermal emission from a tungsten filament are accelerated through voltages of the order of 100 kV , forming the illuminating ...
... light microscope , in which light rays are replaced by a beam of accelerated electrons . Electrons produced by thermal emission from a tungsten filament are accelerated through voltages of the order of 100 kV , forming the illuminating ...
Page 209
... light was analyzed with a Polaroid filter . Figure 14 shows the arrangement of polarizing filters found suitable in ... light - scattering photometer ( Young and Potts , 1963 ) . Polarizing materials most often used in fluorescence are ...
... light was analyzed with a Polaroid filter . Figure 14 shows the arrangement of polarizing filters found suitable in ... light - scattering photometer ( Young and Potts , 1963 ) . Polarizing materials most often used in fluorescence are ...
Page 274
... light intensity falling on the photomultiplier and the frequency bandwidth of the detecting and amplifying system . The noise is largely " shot noise " which is due to changes in the electron flux impinging on the photoanode ( secondary ...
... light intensity falling on the photomultiplier and the frequency bandwidth of the detecting and amplifying system . The noise is largely " shot noise " which is due to changes in the electron flux impinging on the photoanode ( secondary ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone