Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
From inside the book
Results 1-3 of 89
Page 43
... measured length of the shadow cast by particle effective shadow ratio X total magnification ( 11 ) Measurements of the lengths of shadows is a somewhat subjective procedure since , even under ideal conditions , the tips of shadows are ...
... measured length of the shadow cast by particle effective shadow ratio X total magnification ( 11 ) Measurements of the lengths of shadows is a somewhat subjective procedure since , even under ideal conditions , the tips of shadows are ...
Page 157
... measurements of difference spectra . A series of solutions having equal increments in con- centration is used . They are measured one against another , in such a way that the concentration difference between the sample solution and the ...
... measurements of difference spectra . A series of solutions having equal increments in con- centration is used . They are measured one against another , in such a way that the concentration difference between the sample solution and the ...
Page 357
... measured length of the electrodes , taking into account the form of electric field at their ends . He gave the following expression for the corrected length α a 1 = 10 + + [ 1 + 2 log ( 1 + 9 ) ] - π a ( 52 ) where d is the thickness of ...
... measured length of the electrodes , taking into account the form of electric field at their ends . He gave the following expression for the corrected length α a 1 = 10 + + [ 1 + 2 log ( 1 + 9 ) ] - π a ( 52 ) where d is the thickness of ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
45 other sections not shown
Other editions - View all
Common terms and phrases
absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone