Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 406
... patterns are bimodal reaction boundaries . An important feature of the ascending and descending patterns is their marked nonenantiography arising solely from the interaction . Whereas in the ascending pattern the fast - moving peak is ...
... patterns are bimodal reaction boundaries . An important feature of the ascending and descending patterns is their marked nonenantiography arising solely from the interaction . Whereas in the ascending pattern the fast - moving peak is ...
Page 408
... patterns by systematic variation of buffer concentration ( see theoretical patterns presented in Figs . 7 and 8 of Cann and Goad , 1965a ) , ( 2 ) the observed changes in pH across the several peaks in the patterns , ( 3 ) the depend ...
... patterns by systematic variation of buffer concentration ( see theoretical patterns presented in Figs . 7 and 8 of Cann and Goad , 1965a ) , ( 2 ) the observed changes in pH across the several peaks in the patterns , ( 3 ) the depend ...
Page 410
... patterns have also been computed for the other case in which the protein is alkaline to its isoelectric point ( with nega- tive mobilities and the mobility of the complexed protein being smaller than that of the uncomplexed protein ...
... patterns have also been computed for the other case in which the protein is alkaline to its isoelectric point ( with nega- tive mobilities and the mobility of the complexed protein being smaller than that of the uncomplexed protein ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone