Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 83
... peaks appear close to the true positions of the lighter atoms . The peaks associated with the hydrogen atoms are very weak and are lost in the spurious background . The accuracy of the map can be improved by Fourier refinement . In ...
... peaks appear close to the true positions of the lighter atoms . The peaks associated with the hydrogen atoms are very weak and are lost in the spurious background . The accuracy of the map can be improved by Fourier refinement . In ...
Page 408
... peaks but only a single broad descending one ) is shown typically by a variety of proteins in media containing 0.01 M sodium acetate buffer . In addition , the theory accounts for ( 1 ) the progressive changes induced in the patterns by ...
... peaks but only a single broad descending one ) is shown typically by a variety of proteins in media containing 0.01 M sodium acetate buffer . In addition , the theory accounts for ( 1 ) the progressive changes induced in the patterns by ...
Page 409
... peaks . This plateau is maintained until about 10 * seconds . For longer times of electrophoresis , the gradient between the peaks changes sign and the curve becomes slightly depressed below the base line . In other words , the ...
... peaks . This plateau is maintained until about 10 * seconds . For longer times of electrophoresis , the gradient between the peaks changes sign and the curve becomes slightly depressed below the base line . In other words , the ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone