Physical Principles and Techniques of Protein Chemistry, Part 1 |
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Page 9
Phase Contrast Ideally , light or electrons traveling through any portion of a lens
travel equal optical paths ( refractive index X distance ) before reaching the
image . If variations in optical path occur , variations in the intensity of image
points are ...
Phase Contrast Ideally , light or electrons traveling through any portion of a lens
travel equal optical paths ( refractive index X distance ) before reaching the
image . If variations in optical path occur , variations in the intensity of image
points are ...
Page 211
In the first of these , termed phase photometry , a fluorescent sample is irradiated
by a modulated light beam and the phase shift produced as a consequence of
the finite duration of the excited state is measured ( Pringsheim , 1949 ; Steiner ...
In the first of these , termed phase photometry , a fluorescent sample is irradiated
by a modulated light beam and the phase shift produced as a consequence of
the finite duration of the excited state is measured ( Pringsheim , 1949 ; Steiner ...
Page 212
The output from the photomultiplier monitoring the sample cuvette enters a phase
variation unit and then is coupled with the output from the photomultiplier
monitoring the reference source . The two outputs are balanced to approximately
...
The output from the photomultiplier monitoring the sample cuvette enters a phase
variation unit and then is coupled with the output from the photomultiplier
monitoring the reference source . The two outputs are balanced to approximately
...
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Contents
LIST OF CONTRIBUTORS | 2 |
The Enhancement of Contrast | 21 |
The Preservation of Specimens | 35 |
Copyright | |
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absorbance absorption acid appears applied atoms axis birefringence boundary buffer calculated cell charge Chem chromophores complex concentration constant containing contrast corrected corresponding curve dependence determined dielectric difference diffusion dipole direction distribution effects electric electric field electron electrophoresis elution emission energy equation equilibrium example excitation experimental experiments factor flow fluorescence fraction frequency function gel filtration given groups important increase indicates intensity interactions ionic ions length light limited macromolecules measured method microscope migration mobility molecular molecules myosin observed obtained occurs optical particles patterns peaks perturbation phase plot polarization position possible preparation present produced protein range ratio reaction reference relative relaxation respectively rotation scattering separation serum albumin shift shown single solution solvent specimen spectra spectrum strength structure studies technique theoretical theory tion unit usually values volume wavelength weight yield zone