Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
From inside the book
Results 1-3 of 18
Page 5
... Photographic plate ( a ) Light microscope ( b ) Electron microscope . FIG . 1. Comparison of the optical components of ( a ) , the compound light micro- scope and ( b ) , the electron microscope . As shown , each instrument consists of ...
... Photographic plate ( a ) Light microscope ( b ) Electron microscope . FIG . 1. Comparison of the optical components of ( a ) , the compound light micro- scope and ( b ) , the electron microscope . As shown , each instrument consists of ...
Page 34
... PHOTOGRAPHIC METHODS The range of photographic densities present in an electron micrograph may be extended by appropriate photographic processing , with the result that certain features of the image become more evident . Conversely , im ...
... PHOTOGRAPHIC METHODS The range of photographic densities present in an electron micrograph may be extended by appropriate photographic processing , with the result that certain features of the image become more evident . Conversely , im ...
Page 41
... photographic enlargement is required , the photographic grain of the original emulsion will set a limit to resolution . At the same time , excessive magnification should be avoided , since this limits the field of view so that images of ...
... photographic enlargement is required , the photographic grain of the original emulsion will set a limit to resolution . At the same time , excessive magnification should be avoided , since this limits the field of view so that images of ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
45 other sections not shown
Other editions - View all
Common terms and phrases
absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone