Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 232
... rigid sphere whereas the relaxation time ratio will increase only if the molecule becomes asymmetric while remaining completely rigid . The acquisition of rotational degrees of freedom will result in a decrease in ph / po . Consequently ...
... rigid sphere whereas the relaxation time ratio will increase only if the molecule becomes asymmetric while remaining completely rigid . The acquisition of rotational degrees of freedom will result in a decrease in ph / po . Consequently ...
Page 338
... rigid particles . The particle was assumed to be an ellipsoid of revolution and dissolved in a continuous fluid medium . The measurement of the electric birefringence of colloidal solutions in pulsed fields was initiated independently ...
... rigid particles . The particle was assumed to be an ellipsoid of revolution and dissolved in a continuous fluid medium . The measurement of the electric birefringence of colloidal solutions in pulsed fields was initiated independently ...
Page 339
... rigid macromolecules . This method has been applied to tobacco mosaic virus , synthetic polypeptides , and collagen . Holcomb and Tinoco ( 1963 ) extended the birefringence saturation theory to the most general molecular model , and ...
... rigid macromolecules . This method has been applied to tobacco mosaic virus , synthetic polypeptides , and collagen . Holcomb and Tinoco ( 1963 ) extended the birefringence saturation theory to the most general molecular model , and ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone