Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 235
... rotatory criteria . The polarization should decrease regularly with increasing unfolding and approach the value given when the dye is bound to a small structure- less polypeptide . In the latter case , the polarization should be close ...
... rotatory criteria . The polarization should decrease regularly with increasing unfolding and approach the value given when the dye is bound to a small structure- less polypeptide . In the latter case , the polarization should be close ...
Page 263
... rotatory dispersion curve of CT in buffer of pH 6.7 was also measured and com- pared with the O.R.D. curve for CT + PF . Since the two O.R.D. curves were superimposable and since PF alone does not have any appreciable optical rotation ...
... rotatory dispersion curve of CT in buffer of pH 6.7 was also measured and com- pared with the O.R.D. curve for CT + PF . Since the two O.R.D. curves were superimposable and since PF alone does not have any appreciable optical rotation ...
Page 522
... rotatory dispersion conformational changes and , 122-123 , 131 , 137 , 226-231 , 234 a - helix content by , 115 rapid reaction kinetics by , 261-262 , 265 , 283 Order of reaction , determination of , 286 Orientation , of fiber and ...
... rotatory dispersion conformational changes and , 122-123 , 131 , 137 , 226-231 , 234 a - helix content by , 115 rapid reaction kinetics by , 261-262 , 265 , 283 Order of reaction , determination of , 286 Orientation , of fiber and ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone