Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 7
... scattering and focus effects are the principal sources of con- trast . For very small objects , phase - contrast effects may be important . 2. Differential Scattering Contrast Electron scattering by the background ( i.e. , by the ...
... scattering and focus effects are the principal sources of con- trast . For very small objects , phase - contrast effects may be important . 2. Differential Scattering Contrast Electron scattering by the background ( i.e. , by the ...
Page 56
... scattering contrast would be reduced . The development of high resolution electron phase contrast systems thus seems essential , since contrast would then result from scattering within the aperture of an ideal lens . Exploitation of ...
... scattering contrast would be reduced . The development of high resolution electron phase contrast systems thus seems essential , since contrast would then result from scattering within the aperture of an ideal lens . Exploitation of ...
Page 165
Sydney J. Leach. scattering . If scattering is present a plot of log D vs. log λ will be linear in the 400-310 mμ region ( Fig . 26 ) . Experimentally , the slope of such a plot is rarely 4 , as predicted for scattering from particles ...
Sydney J. Leach. scattering . If scattering is present a plot of log D vs. log λ will be linear in the 400-310 mμ region ( Fig . 26 ) . Experimentally , the slope of such a plot is rarely 4 , as predicted for scattering from particles ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone