Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 202
... curve , it is less well known that the type of grating ( or prism ) in the emission monochromator is equally ... scatter peak at 278 mu , which could be subtracted by using a buffer blank . Scatter signals en- countered in obtaining ...
... curve , it is less well known that the type of grating ( or prism ) in the emission monochromator is equally ... scatter peak at 278 mu , which could be subtracted by using a buffer blank . Scatter signals en- countered in obtaining ...
Page 213
... curve is exponential if only a single fluorescent species is present . Nonexponential decay is indicative of the ... scattering solution ( Chen et al . , 1967 ) . The other channel of the oscilloscope is used to display signals from a " decay ...
... curve is exponential if only a single fluorescent species is present . Nonexponential decay is indicative of the ... scattering solution ( Chen et al . , 1967 ) . The other channel of the oscilloscope is used to display signals from a " decay ...
Page 466
... scatter inherent in manual differentiation of elution profiles is obviously ... plot of the quantity ( Ca + Ct — Co ) vs. V , where ca and ct are the solute con ... curve obtained by plotting differences between absorbancy readings of ...
... scatter inherent in manual differentiation of elution profiles is obviously ... plot of the quantity ( Ca + Ct — Co ) vs. V , where ca and ct are the solute con ... curve obtained by plotting differences between absorbancy readings of ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone