Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 108
... Shift from pH 26 ( cm - 1 ) Spectrum Phenylalanine at pH 2 Phenylalanine at pH 12 Phenylalanine in gelatin sol ... shift . The observed red shift of the phenylalanine spectrum compared to that of benzene means that the electronic energy ...
... Shift from pH 26 ( cm - 1 ) Spectrum Phenylalanine at pH 2 Phenylalanine at pH 12 Phenylalanine in gelatin sol ... shift . The observed red shift of the phenylalanine spectrum compared to that of benzene means that the electronic energy ...
Page 110
... shift . Two other interactions between chromophores and solvent occur . The first and most ubiquitous is the London dispersion interaction , the polari- zation of the electron distribution of solvent molecules by rapid alterations in ...
... shift . Two other interactions between chromophores and solvent occur . The first and most ubiquitous is the London dispersion interaction , the polari- zation of the electron distribution of solvent molecules by rapid alterations in ...
Page 111
Sydney J. Leach. TABLE V DIRECTION OF SOLVENT - PRODUCED WAVELENGTH SHIFT " Shifts produced from : Polariza- Change in tion by Polariza- tion by Chro- chromophore transi- Orienta- perma- Vibrational mo- Sol- dipole Case phore vent moment ...
Sydney J. Leach. TABLE V DIRECTION OF SOLVENT - PRODUCED WAVELENGTH SHIFT " Shifts produced from : Polariza- Change in tion by Polariza- tion by Chro- chromophore transi- Orienta- perma- Vibrational mo- Sol- dipole Case phore vent moment ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone