Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 26
... significant regular structures at some positions . Thus , an apparent substructure of a shadowed particle cannot be considered significant un- less it is clearly and reproducibly differentiable from the surrounding background . Any ...
... significant regular structures at some positions . Thus , an apparent substructure of a shadowed particle cannot be considered significant un- less it is clearly and reproducibly differentiable from the surrounding background . Any ...
Page 47
... significant vari- ations do occur , in contrast to the highly constant spacings between rulings of a diffraction grating or atoms of a crystal . Polystyrene spheres in fact serve two quite different purposes in the electron microscopy ...
... significant vari- ations do occur , in contrast to the highly constant spacings between rulings of a diffraction grating or atoms of a crystal . Polystyrene spheres in fact serve two quite different purposes in the electron microscopy ...
Page 78
... significant errors are introduced into the finer details of the electron density map . D. SERIES TERMINATION ERRORS The errors that would be introduced by excessive artificial sharpening are very similar to those caused by an abrupt ...
... significant errors are introduced into the finer details of the electron density map . D. SERIES TERMINATION ERRORS The errors that would be introduced by excessive artificial sharpening are very similar to those caused by an abrupt ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone