Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 34
... specific positive staining of proteins and other molecules in thin tissue sections , is an increasingly important technique . Materials which combine with antibodies or with specific chemical reagents can be localized in tissues , as ...
... specific positive staining of proteins and other molecules in thin tissue sections , is an increasingly important technique . Materials which combine with antibodies or with specific chemical reagents can be localized in tissues , as ...
Page 309
... specific conductivity κ of the solution at each frequency K C = εer ( A / l ) G = = k ( A / l ) ( 62 ) ( 63 ) where C is the corrected capacity , G is the conductance , and e , is the dielectric constant of free space which is 8.85 × 10 ...
... specific conductivity κ of the solution at each frequency K C = εer ( A / l ) G = = k ( A / l ) ( 62 ) ( 63 ) where C is the corrected capacity , G is the conductance , and e , is the dielectric constant of free space which is 8.85 × 10 ...
Page 349
... specific parallel dichroism , ( A || — A ) / A , or the specific perpendicular dichroism , ( AA ) / A , from the experi- mental point of view , where A is the absorbance in the absence of an electric field ( Paulson , 1965 ) . It is to ...
... specific parallel dichroism , ( A || — A ) / A , or the specific perpendicular dichroism , ( AA ) / A , from the experi- mental point of view , where A is the absorbance in the absence of an electric field ( Paulson , 1965 ) . It is to ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone