Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
From inside the book
Results 1-3 of 48
Page 7
... specimen . This mechanism of contrast is in fact normally taken for granted . Absorption contrast is enhanced in stained specimens . To a more limited degree ... Specimen } Specimen Lens 1. ELECTRON MICROSCOPY OF GLOBULAR PROTEINS 7.
... specimen . This mechanism of contrast is in fact normally taken for granted . Absorption contrast is enhanced in stained specimens . To a more limited degree ... Specimen } Specimen Lens 1. ELECTRON MICROSCOPY OF GLOBULAR PROTEINS 7.
Page 25
... specimen vertical distance of evaporation source above specimen ( 3 ) In principle , this quantity should be equal to the effective shadow ratio , which is length of shadow cast by specimen height of specimen contour above substrate ( 4 ) ...
... specimen vertical distance of evaporation source above specimen ( 3 ) In principle , this quantity should be equal to the effective shadow ratio , which is length of shadow cast by specimen height of specimen contour above substrate ( 4 ) ...
Page 46
... Specimens An ideal calibration specimen contains regular spacings which are known precisely by means which are independent of electron microscopy , and which , ideally , should be comparable in size to the objects to be measured . The ...
... Specimens An ideal calibration specimen contains regular spacings which are known precisely by means which are independent of electron microscopy , and which , ideally , should be comparable in size to the objects to be measured . The ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
45 other sections not shown
Other editions - View all
Common terms and phrases
absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone