Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 182
... speed of Brownian rotation . If no appreciable rotation occurs during the lifetime of the excited state , as in the case of a highly viscous medium , this angle , which determines the polarization , will retain its initial value . In ...
... speed of Brownian rotation . If no appreciable rotation occurs during the lifetime of the excited state , as in the case of a highly viscous medium , this angle , which determines the polarization , will retain its initial value . In ...
Page 197
... speed motors which in turn are directly coupled to potentiometers controlling the reference voltage of a mercury battery supplying the signal to the x - axis of an x - y recorder , while the amplified photomultiplier signal is fed to ...
... speed motors which in turn are directly coupled to potentiometers controlling the reference voltage of a mercury battery supplying the signal to the x - axis of an x - y recorder , while the amplified photomultiplier signal is fed to ...
Page 215
... speed of light , a is the effective molecular radius , pe and μ , are the dipole mo- ments of the excited and ground states of the fluorescent molecule , and Af is the reorientation polarization factor defined by Af = D - 1 2D + 1 n2 ...
... speed of light , a is the effective molecular radius , pe and μ , are the dipole mo- ments of the excited and ground states of the fluorescent molecule , and Af is the reorientation polarization factor defined by Af = D - 1 2D + 1 n2 ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone