Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 12
... Structure within the film may thus easily be confused with fine structure in the supported particles . High - resolution microscopy reveals structure in carbon films down to the finest level observable , which is about 5 Å . The ...
... Structure within the film may thus easily be confused with fine structure in the supported particles . High - resolution microscopy reveals structure in carbon films down to the finest level observable , which is about 5 Å . The ...
Page 79
... structure amplitude information by giving F ( hkl ) | the value it would have if the atoms were randomly distributed throughout the whole crystal ( Srinivasan , 1961 ) . The first synthesis bears no obvious relation to the structure ...
... structure amplitude information by giving F ( hkl ) | the value it would have if the atoms were randomly distributed throughout the whole crystal ( Srinivasan , 1961 ) . The first synthesis bears no obvious relation to the structure ...
Page 89
... structure factor Fp of a protein reflection is known , the structure factor of a closely related structure FPD will be the vector sum of Fp and the structure factor FD of the difference between the two structures . The magnitude and ...
... structure factor Fp of a protein reflection is known , the structure factor of a closely related structure FPD will be the vector sum of Fp and the structure factor FD of the difference between the two structures . The magnitude and ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone