Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 60
... unit cell the fractional coordinates of the jth atom in the unit cell indices of a reflection ; coordinates of a reciprocal lattice point . h is also used for the axial rise per unit in describing helical structures lengths of the ...
... unit cell the fractional coordinates of the jth atom in the unit cell indices of a reflection ; coordinates of a reciprocal lattice point . h is also used for the axial rise per unit in describing helical structures lengths of the ...
Page 63
... unit cell in three dimensions as shown in Fig . 2. The unit cell is characterized by the lengths a , b , and c of its edges and the angles Z α ୪ FIG . 2. A crystal may be regarded as being built up by the three - dimensional repetition ...
... unit cell in three dimensions as shown in Fig . 2. The unit cell is characterized by the lengths a , b , and c of its edges and the angles Z α ୪ FIG . 2. A crystal may be regarded as being built up by the three - dimensional repetition ...
Page 67
... unit cell is known , a second important property of the crystal termed the space group can usually be deduced by looking for regular gaps , called systematic absences , in the pattern of reflections obtained from the crystal . The space ...
... unit cell is known , a second important property of the crystal termed the space group can usually be deduced by looking for regular gaps , called systematic absences , in the pattern of reflections obtained from the crystal . The space ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone