Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
From inside the book
Results 1-3 of 43
Page 182
... viscous medium , this angle , which determines the polarization , will retain its initial value . In this case the ... viscosity is possible provided that the following assumptions are valid : 1. All azimuthal orientations of the ...
... viscous medium , this angle , which determines the polarization , will retain its initial value . In this case the ... viscosity is possible provided that the following assumptions are valid : 1. All azimuthal orientations of the ...
Page 232
... viscosity . Both procedures of altering the viscosity may lead to spurious values of the relaxation time . Increasing the temperature may introduce new degrees of rotational freedom in the dye , whereas preferential binding of one of ...
... viscosity . Both procedures of altering the viscosity may lead to spurious values of the relaxation time . Increasing the temperature may introduce new degrees of rotational freedom in the dye , whereas preferential binding of one of ...
Page 321
... viscosity of the solvent . Takashima ( 1962 ) Relaxation time ( 10-6 sec ) 1.5 1.0 0.5- 2.0 T O a b 3 5 7 9 Viscocity ( centipoise ) FIG . 10. Dependence of the relaxation time of proteins on the viscosity of the solvent . The ordinate ...
... viscosity of the solvent . Takashima ( 1962 ) Relaxation time ( 10-6 sec ) 1.5 1.0 0.5- 2.0 T O a b 3 5 7 9 Viscocity ( centipoise ) FIG . 10. Dependence of the relaxation time of proteins on the viscosity of the solvent . The ordinate ...
Contents
Electron Microscopy of Globular Proteins | 2 |
The Enhancement of Contrast | 21 |
The Preservation of Specimens | 35 |
Copyright | |
44 other sections not shown
Other editions - View all
Common terms and phrases
absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone