Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 40
... weight means that measurement by electron microscopy is not a precise method for obtaining molecular weights of smaller macromolecules . For very large particles , with molecular weights in the millions , estimates of molecular weights ...
... weight means that measurement by electron microscopy is not a precise method for obtaining molecular weights of smaller macromolecules . For very large particles , with molecular weights in the millions , estimates of molecular weights ...
Page 468
... weight - average molec- ular weight data to define the molecular weight of the monomeric unit in systems undergoing rapid , reversible polymerization ( Winzor and Scheraga , 1964 ) . This section summarizes the relevant practical ...
... weight - average molec- ular weight data to define the molecular weight of the monomeric unit in systems undergoing rapid , reversible polymerization ( Winzor and Scheraga , 1964 ) . This section summarizes the relevant practical ...
Page 472
... weight of an enzyme at enzymic concentra- tion levels may often represent such a situation ( Section IV , C ) . C. MONOMER MOLECULAR WEIGHTS OF RAPIDLY POLYMERIZING PROTEINS In a solute system comprising monomer in rapidly established ...
... weight of an enzyme at enzymic concentra- tion levels may often represent such a situation ( Section IV , C ) . C. MONOMER MOLECULAR WEIGHTS OF RAPIDLY POLYMERIZING PROTEINS In a solute system comprising monomer in rapidly established ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone