Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 421
... zone electrophoretic patterns as moving - boundary patterns . In particular , cognizance must be taken of the fact that multiple zones need not necessarily indicate inherent heterogeneity . As will be shown below , a single ...
... zone electrophoretic patterns as moving - boundary patterns . In particular , cognizance must be taken of the fact that multiple zones need not necessarily indicate inherent heterogeneity . As will be shown below , a single ...
Page 432
... zone electrophoresis in a density gradient of sucrose or D2O than by moving - boundary electrophoresis . Zone electrophoresis in a den- sity gradient also holds promise as a powerful tool for quantitative characterization of certain ...
... zone electrophoresis in a density gradient of sucrose or D2O than by moving - boundary electrophoresis . Zone electrophoresis in a den- sity gradient also holds promise as a powerful tool for quantitative characterization of certain ...
Page 436
... zone patterns are presented in Figs . 26a and 26b ; whereas the protein gave a single zone in phosphate buffer , two zones were obtained in phosphate - borate , the faster - moving zone being the major one . In order to determine ...
... zone patterns are presented in Figs . 26a and 26b ; whereas the protein gave a single zone in phosphate buffer , two zones were obtained in phosphate - borate , the faster - moving zone being the major one . In order to determine ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone