Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 266
... fit to the data . The requirement that two independent sets 2 3 4 PM ཐ་ FIG . 8. Differential spectrophotometry using tandem cells . The separate compart- ments contain : 1 , CT ; 2 , PF ; 3 , CT + PF ; 4 , buffer ( not absorbing in the ...
... fit to the data . The requirement that two independent sets 2 3 4 PM ཐ་ FIG . 8. Differential spectrophotometry using tandem cells . The separate compart- ments contain : 1 , CT ; 2 , PF ; 3 , CT + PF ; 4 , buffer ( not absorbing in the ...
Page 313
... fits the Debye theory within experimental error . The Cole - Cole plot ( Cole and Cole , 1941 ) is 88 86 Dielectric constant 30 80 78 α 100 kc I Mc 8 επ 88 86 10 Mc Frequency Cole Cole plot Ο 60 +88 84 82 80 78 100 Mc ო ε ' FIG . 6 ...
... fits the Debye theory within experimental error . The Cole - Cole plot ( Cole and Cole , 1941 ) is 88 86 Dielectric constant 30 80 78 α 100 kc I Mc 8 επ 88 86 10 Mc Frequency Cole Cole plot Ο 60 +88 84 82 80 78 100 Mc ო ε ' FIG . 6 ...
Page 342
... fit a theoretical curve of 150 / ( ẞ2 + 2y ) vs. | ẞ2 + 2y if the scale factor b2 + 2c is chosen properly . A more convenient method of fitting is to prepare a set of curves of 150 / ( B2 + 2y ) as ordinate vs. log ( B2 + 2y ) as ...
... fit a theoretical curve of 150 / ( ẞ2 + 2y ) vs. | ẞ2 + 2y if the scale factor b2 + 2c is chosen properly . A more convenient method of fitting is to prepare a set of curves of 150 / ( B2 + 2y ) as ordinate vs. log ( B2 + 2y ) as ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Dielectric Properties of Proteins 7 | 7 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone