Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 119
... difference spectrum will have the wave- length dependence of the difference absorption coefficient ( Fig . 4 ) . Sim- ilarly , at any fixed wavelength and chromophore concentration , the optical density difference measured as a function ...
... difference spectrum will have the wave- length dependence of the difference absorption coefficient ( Fig . 4 ) . Sim- ilarly , at any fixed wavelength and chromophore concentration , the optical density difference measured as a function ...
Page 124
... difference spectrum , defined as Ae ( λ ) = € ( λ — Aλ ) — € ( λ ) , is - Ae ( A ) ~ AX ( de / dλ ) ( 12 ) This approximation will be best at the wavelength at which the differ- ence spectrum is a maximum , since at this wavelength ( de ...
... difference spectrum , defined as Ae ( λ ) = € ( λ — Aλ ) — € ( λ ) , is - Ae ( A ) ~ AX ( de / dλ ) ( 12 ) This approximation will be best at the wavelength at which the differ- ence spectrum is a maximum , since at this wavelength ( de ...
Page 158
... difference of 0.02 can be measured with 1 % accuracy . If spectra are recorded slowly , using a long time constant in order to im- prove signal - to - noise ratio , it should be established that the zero drift of the instrument is not ...
... difference of 0.02 can be measured with 1 % accuracy . If spectra are recorded slowly , using a long time constant in order to im- prove signal - to - noise ratio , it should be established that the zero drift of the instrument is not ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Dielectric Properties of Proteins 7 | 7 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone