Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 209
... polarizing filters found suitable in measuring polarization using a spectrofluorometer ( Chen and Bow- man , 1965 ) . In this case , a correction factor must be applied to compen- sate for a degree of polarization added by the mirrors ...
... polarizing filters found suitable in measuring polarization using a spectrofluorometer ( Chen and Bow- man , 1965 ) . In this case , a correction factor must be applied to compen- sate for a degree of polarization added by the mirrors ...
Page 210
... polarization spectrum will have structure , but the polarization in the emission band is normally constant ( Feofilov , 1961 ) . Important exceptions to the constancy of the emission polarization spectrum are indole ( Zimmermann and ...
... polarization spectrum will have structure , but the polarization in the emission band is normally constant ( Feofilov , 1961 ) . Important exceptions to the constancy of the emission polarization spectrum are indole ( Zimmermann and ...
Page 222
... Polarization . values which were smaller than those of tyrosine were observed in ribonuclease , insulin , and zein at -70 ° C in propylene glycol ( Weber , 1960b ) . The excitation polarization spectrum of tryptophan is more complex ...
... Polarization . values which were smaller than those of tyrosine were observed in ribonuclease , insulin , and zein at -70 ° C in propylene glycol ( Weber , 1960b ) . The excitation polarization spectrum of tryptophan is more complex ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Dielectric Properties of Proteins 7 | 7 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone