Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
From inside the book
Results 1-3 of 90
Page 123
... spectrum obeyed Beer's law , so that the molar difference absorption coefficient was concentra- tion - independent ; ( 3 ) solvent perturbation experiments using methanol , ethylene glycol , polyethylene glycol , and glycerol ...
... spectrum obeyed Beer's law , so that the molar difference absorption coefficient was concentra- tion - independent ; ( 3 ) solvent perturbation experiments using methanol , ethylene glycol , polyethylene glycol , and glycerol ...
Page 124
... spectrum , defined as Ae ( λ ) = € ( λ — Aλ ) — € ( λ ) , is - Ae ( A ) ~ AX ( de / dλ ) ( 12 ) This approximation will be best at the wavelength at which the differ- ence spectrum is a maximum , since at this wavelength ( de / dλ ) is ...
... spectrum , defined as Ae ( λ ) = € ( λ — Aλ ) — € ( λ ) , is - Ae ( A ) ~ AX ( de / dλ ) ( 12 ) This approximation will be best at the wavelength at which the differ- ence spectrum is a maximum , since at this wavelength ( de / dλ ) is ...
Page 155
... spectrum viewed by the slit . The slit width extends from a to b in each diagram . The measured value of the absorbance , to a first approximation , is the average absorption to the left of λ plus the average absorption to the right of ...
... spectrum viewed by the slit . The slit width extends from a to b in each diagram . The measured value of the absorbance , to a first approximation , is the average absorption to the left of λ plus the average absorption to the right of ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Dielectric Properties of Proteins 7 | 7 |
The Enhancement of Contrast | 21 |
Copyright | |
60 other sections not shown
Other editions - View all
Common terms and phrases
absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone