Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 460
... Volume ( ml ) 60 80 FIG . 2. Gel filtration of ovalbumin on a 90 ml ( 19.5 × 2.4 cm ) column of Sephadex G - 100 equilibrated with phosphate buffer ( pH 6.8 , ionic strength 0.1 ) . ( a ) Zonal profile resulting from application of 3 ml ...
... Volume ( ml ) 60 80 FIG . 2. Gel filtration of ovalbumin on a 90 ml ( 19.5 × 2.4 cm ) column of Sephadex G - 100 equilibrated with phosphate buffer ( pH 6.8 , ionic strength 0.1 ) . ( a ) Zonal profile resulting from application of 3 ml ...
Page 476
... volume ) required for migration through the void volume ( V. ) , the boundary center requiring the elution volume ( Ve ) for this purpose . It is therefore argued that the standard deviation . of the concentration - effluent volume ...
... volume ) required for migration through the void volume ( V. ) , the boundary center requiring the elution volume ( Ve ) for this purpose . It is therefore argued that the standard deviation . of the concentration - effluent volume ...
Page 489
... volume V ' inter- mediate between VA and Vc , and a spread boundary with an elution volume VA . Gilbert and Jenkins ( 1959 ) have shown that K = z / mcα ( 38a ) where 2 = - { ( VA — V ' ) / ( V ' — Vc ) } ' - ( 38b ) These equations may ...
... volume V ' inter- mediate between VA and Vc , and a spread boundary with an elution volume VA . Gilbert and Jenkins ( 1959 ) have shown that K = z / mcα ( 38a ) where 2 = - { ( VA — V ' ) / ( V ' — Vc ) } ' - ( 38b ) These equations may ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Dielectric Properties of Proteins 7 | 7 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone