Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 204
... Yield The quantum yield ( or fluorescence efficiency ) Q is defined as the ratio of the number of photons emitted to the number absorbed ; it is important for a number of reasons indicated in Section I of this chapter . The quantum ...
... Yield The quantum yield ( or fluorescence efficiency ) Q is defined as the ratio of the number of photons emitted to the number absorbed ; it is important for a number of reasons indicated in Section I of this chapter . The quantum ...
Page 205
... yield standards is given in Table I. Of these , quinine in sulfuric acid has been used probably by more laboratories than any TABLE I QUANTUM YIELD STANDARDS Compound Solvent Reference Acridone Ethanol 0.83 Melhuish ( 1961 ) Anthracene ...
... yield standards is given in Table I. Of these , quinine in sulfuric acid has been used probably by more laboratories than any TABLE I QUANTUM YIELD STANDARDS Compound Solvent Reference Acridone Ethanol 0.83 Melhuish ( 1961 ) Anthracene ...
Page 220
Sydney J. Leach. the quantum yield does not correspond to that of the helix - coil transition . ( Wada and Ueno , 1964 ) . An important contribution to the low yield at neutral pH may be proton transfer from the excited tyrosyl to ...
Sydney J. Leach. the quantum yield does not correspond to that of the helix - coil transition . ( Wada and Ueno , 1964 ) . An important contribution to the low yield at neutral pH may be proton transfer from the excited tyrosyl to ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Dielectric Properties of Proteins 7 | 7 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration conformational changes contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic Phys plot polarization polymer produced protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone